Fig. 7.

Effect of nucleotide polymorphism at positions 1765 to 1771 on probe performance. (A) A noncontiguous Megablast search was performed using a query sequence of HBV nucleotides 1734 to 1793. A total of 8,227 sequences were compiled here for nucleotides 1762 to 1771, excluding the ones with incomplete and uncertain sequence readings. The sequence patterns are sorted according to their appearance frequencies in the GenBank, and their percent representation in the total number of sequences is listed. The area covered by the probe is highlighted. (B) Melting analysis of the different sequence patterns listed in panel A. Patterns 7 and 8 (data not shown) showed the same melting curves as patterns 3 and 5, respectively.