Table 1.
Cross-reactivity of the multiplex blaKPC/RNase P qPCR assay
| Bacterial strain | CT reading | blaKPC/RNase P qPCR result |
|---|---|---|
| K. pneumoniae ATCC 13883 | Undetermineda | Negative |
| K. pneumoniae ATCC 700603 (ESBLb) | Undetermined | Negative |
| E. coli ATCC 25922 | Undetermined | Negative |
| E. coli ATCC 35218 | Undetermined | Negative |
| K. pneumoniae (AmpC) | Undetermined | Negative |
| E. coli (AmpC) | Undetermined | Negative |
| Acinetobacter baumannii | Undetermined | Negative |
| Citrobacter koseri | Undetermined | Negative |
| Enterobacter species | Undetermined | Negative |
| Serratia marcescens | Undetermined | Negative |
| Salmonella species | Undetermined | Negative |
| Salmonella paratyphi type A | Undetermined | Negative |
| Shigella species | Undetermined | Negative |
| Stenotrophomonas maltophilia | Undetermined | Negative |
| Proteus mirabilis | Undetermined | Negative |
| Pseudomonas aeruginosa (MDRc) | Undetermined | Negative |
| Morganella morganii | Undetermined | Negative |
| Providencia species | Undetermined | Negative |
| Aeromonas hydrophila (MDR) | Undetermined | Negative |
| Staphylococcus aureus (methicillin resistant) | Undetermined | Negative |
| Staphylococcus aureus (methicillin susceptible) | Undetermined | Negative |
| Streptococcus agalactiae | Undetermined | Negative |
| Enterococcus species | Undetermined | Negative |
| Chryseobacterium species | Undetermined | Negative |
| Bacteroides species | Undetermined | Negative |
| E. coli (blaNDM) | Undetermined | Negative |
| Providencia rettgeri (blaNDM) | Undetermined | Negative |
| Candida albicans ATCC 14053 | Undetermined | Negative |
| Candida parapsilosis ATCC 22019 | Undetermined | Negative |
a
Undetermined, the sample's fluorescence reading was below the threshold cutoff value of 50 cycles.
b
ESBL, extended-spectrum β-lactamase producer.
c
MDR, multidrug resistant.