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Primer annealing sites. Sequences of both plasmids rs12979860 (A) and rs8099917 (B), along with the allele-specific primers, are depicted. The conserved nucleotide positions throughout the alignment are indicated by dots. The target SNP position is designated by asterisks. Solid arrows represent the positions for the melt-MAMA (forward) primer, and the dashed arrows depict the locations of the corresponding reverse primers.
