Fig. 1.

Schematic diagram of KSHV LANA and LANA deletion mutants used in this investigation. Indicated are the proline-rich region (P), the aspartate- and glutamate-rich region (DE), the glutamine- and glutamate-rich region (Q) and the putative leucine zipper (LZ). The DE, Q, and LZ regions all contain repeat elements. The shaded region represents the N-terminal nuclear localization (NLS) signal. C-terminal LANA can also localize to nuclei but an NLS has not been precisely mapped. Amino acids 5 to 13 mediate chromosome association through interaction with histones H2A/H2B. Amino acids 996 to 1139 contain the TR DNA binding, self-association, and chromosome association functions. Capabilities for TR DNA binding, chromosome association, DNA replication, and episome persistence for each of the constructs are summarized at the right. Fractions are the numbers of G418-resistant cell lines containing episomes over the total number of G418-resistant cell lines assayed by Gardella analysis, and percentages are shown in parentheses.