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Induction of target gene expression by RRV ORF57. Plasmids encoding RRV c.o. gH, gH, c.o. gL, and gL were cotransfected with either the empty or the ORF57 expression plasmid, and the levels of protein expressed were measured at 23 (A) or 36 h (B) posttransfection by immunoblotting. The top band, but not the bottom band, of the gH doublet appears to be an O-glycosylated form of gH on the basis of Jacalin reactivity (38).
