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Accumulation of target RNAs by RRV ORF57. Plasmids encoding RRV c.o. gH, gH, c.o. gL, and gL were cotransfected with either the empty or the ORF57 expression vector. Total RNA was isolated at 23 h (A) or 36 h (B) posttransfection, and an equal amount of total RNA (4 μg) was loaded into each lane. The levels of each target RNA were measured by Northern blotting. The 28S and 18S rRNA bands from ethidium bromide staining are shown as loading controls.
