Fig. 8.

Cellular localization of gH RNA. A plasmid encoding c.o. gH was transfected into HEK293T cells together with either the empty or the RRV ORF57 expression vector. Cells were harvested at 26 or 36 h posttransfection and fractionated to separate the cytoplasmic (C) and nuclear (N) RNA. (A) Cytoplasmic RNA (2.2 μg) and nuclear RNA (1.5 μg) were loaded into each corresponding lane, and c.o. gH RNA was detected by Northern blotting. The 28S and 18S as well as the 45S and 32S rRNA bands from ethidium bromide staining are shown as loading and fractionation controls. (B) The intensity of each band (in arbitrary units [AU]) was analyzed using Image Gauge (Fuji Film) and was graphically represented.