Table 1.
Virus isolation results after inoculation of ferrets with H1N1 isolates
Virus | Inoculum dose (TCID50/ml) | Virus detection (no. of positive animals/total no. tested [titers]) by sample typea |
|||||
---|---|---|---|---|---|---|---|
Rectal swab at: |
Other at day 3 |
||||||
Day 3 | Day 5 | Small intestine | Olfactory bulb | Lung | Nasal washes | ||
A/California/4/09 | 102 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | 6/6 (6.7, 6.4, 6.4, >6, >6, 5.4) |
A/New Caledonia/20/99 | 102 | 1/3 | 0/3 | 0/3 | 0/3 | 0/3 | 6/6 (6.0, 5.7, 5.4, 5.4, 5.0, 5.0) |
A/Swine/Iowa/15/30 | 102 | 5/6 | 3/3 | 0/3 | 0/3 | 2/3b (<1c, 3.7, 4) | 6/6 (6.4, 6.2, 5.4, 5.4, 5.2, 4.9) |
A/Mallard/MN/A108-2355/08 | 102 | 3/6 | 2/3 | 0/3 | 0/3 | 0/3 | 2/6 (3.2, 2.7, <1, <1, <1, <1) |
Titers are expressed as log10 TCID50/ml.
Confirmed by real-time PCR.
Limit of detection.