Fig. 1.

TGF-β1 induces EBV lytic gene expression in MutuI cells. (A) Immunoblots showing IE BZLF1 and E BMRF1 protein levels following treatment with TGF-β1. MutuI and MutuIII cells were incubated for the indicated times with or without (−) 100 pM TGF-β1. Cell lysates were prepared, and relative protein levels of BZLF1, BMRF1, and α-tubulin (as an internal control) were determined by immunoblot analysis. (B) Percentage of MutuI cells containing the EBV BMRF1 protein at various times after the addition of 100 pM TGF-β1. Cells were harvested at the indicated times and analyzed by FACS for the percentage of BMRF1-positive ones. Bars indicate means ± standard errors of the means (SEM) from assays performed in triplicate on three separate occasions. Significant differences (P < 0.05) are indicated with an asterisk.