Fig. 2.

TGF-β1 secreted by T cells reactivates EBV in MutuI cells. Immunoblot showing relative levels of BZLF1 protein after culture of the indicated cells for 48 h under the indicated conditions. Whole-cell extracts were prepared and BZLF1 protein was assayed by immunoblot analysis, with α-tubulin serving as an internal control. Lane 5, cells cultured in the presence of 100 pM TGF-β1; lane 6, cells cultured in the presence of 2.5 μg TGF-β neutralizing antibody; other lanes, cells cultured in medium containing neither added TGF-β nor antibody.