Fig. 6.

ChIP assay showing TGF-β1 induces Smad4 binding to Zp. MutuI cells were incubated for 30 min in the presence (+) or absence (−) of 100 pM TGF-β1. Cells then were processed for ChIP and immunoprecipitated with a Smad4-specific antibody or IgG as a control. Recovered DNA was amplified by PCR with primers specific to the nt −221 to +30 region of Zp (15 cycles), the promoter region of the cellular Smad7 gene (25 cycles) as a positive control, and an EBV sequence located 4.8 kbp upstream of Zp (15 cycles) as a negative control. The input lanes contained 10% of the amount of DNA included in the IPs.