Table 3.
Single virion analysis of homozygous and heterozygous particles with 6G and 6C DIS mutants
| Combination and expt | No. of CeFP particles analyzed | % of particles that were CeFP+ and: |
RNA labeling efficiencya (%) | ||
|---|---|---|---|---|---|
| YFP+ | mCherry+ | YFP+ and mCherry+ | |||
| 2-6G-BSLb + Bgl-mCherry | |||||
| Expt 1 | 2,488 | 0.0 | 92.9 | 0.0 | 92.9 |
| Expt 2 | 2,071 | 0.0 | 92.8 | 0.0 | 92.8 |
| Expt 3 | 1,405 | 0.1 | 93.1 | 0.0 | 93.2 |
| Expt 4 | 3,267 | 0.0 | 91.0 | 0.0 | 91.0 |
| 2-6G-BSL + MS2-YFP | |||||
| Expt 1 | 1,659 | 1.1 | 0.0 | 0.0 | 1.1 |
| Expt 2 | 3,890 | 0.9 | 0.0 | 0.0 | 0.9 |
| Expt 3 | 2,810 | 0.5 | 0.0 | 0.0 | 0.5 |
| Expt 4 | 4,037 | 0.7 | 0.0 | 0.0 | 0.7 |
| 2-6C-MSL + MS2-YFP | |||||
| Expt 1 | 826 | 91.8 | 0.0 | 0.0 | 91.8 |
| Expt 2 | 873 | 90.0 | 0.0 | 0.0 | 90.0 |
| Expt 3 | 1,060 | 91.4 | 0.0 | 0.0 | 91.4 |
| Expt 4 | 824 | 91.3 | 0.0 | 0.1 | 91.4 |
| Expt 5 | 1,474 | 91.3 | 0.0 | 0.0 | 91.3 |
| 2-6C-MSL + Bgl-mCherry | |||||
| Expt 1 | 1,401 | 0.0 | 0.0 | 0.0 | 0.0 |
| Expt 2 | 1,671 | 0.0 | 0.0 | 0.0 | 0.1 |
| Expt 3 | 1,734 | 0.0 | 0.0 | 0.0 | 0.0 |
| 2-6G-BSL + 2-6C-MSL + Bgl-mCherry + MS2-YFP | |||||
| Expt 1 | 773 | 12.9 | 21.5 | 57.8 | 92.2 |
| Expt 2 | 1,336 | 17.5 | 18.9 | 57.0 | 93.5 |
| Expt 3 | 887 | 14.2 | 20.6 | 57.2 | 92.0 |
| Expt 4 | 1,638 | 18.7 | 18.4 | 54.9 | 92.1 |
a
Calculated by adding the values from the previous three columns.
b
For simplicity, the “GagCeFP” portions of all the construct names were deleted; for example, 2-6G-BSL is 2-6G-GagCeFP-BSL.