Fig. 3.

TAP purification of UL25-NTAP and UL17-CTAP. Vero cells were infected with HSV-1 recombinant viruses expressing the indicated TAP-tagged protein or with wild-type (KOS) virus. (A) Following TAP purification, SDS-PAGE analysis (silver stain) was done on the proteins eluted from the KOS, UL25-NTAP, and UL17-CTAP calmodulin column. (B) Western blots (antibodies used listed below each blot) demonstrating the presence of the TAP-tagged proteins along with proteins that copurify with the TAP-tagged proteins. Protein standards (kDa) are shown on the left of each gel or blot.