Fig. 2.
Specific sequences in loop 1 of the TRIM5αAGM(Tan) RING domain are critical for SIVmac restriction. (A) The sequences of the RING domains of TRIM5α proteins from African green monkeys (tantalus [Tan] subspecies), humans, rhesus monkeys, and squirrel monkeys are aligned. The amino acid residue numbers are based on those of TRIM5αAGM(Tan). Areas where sequence differences exist are highlighted with gray boxes. The dots above the sequence indicate cysteine and histidine residues involved in zinc coordination. The locations of loops 1 and 2 are shown. (B to F) HeLa (B to E) or Cf2Th (F) cells, either transduced with the empty LPCX vector or expressing TRIM5αAGM(Pyg) or the indicated TRIM5αAGM(Tan) variants, were incubated with different amounts of recombinant VSV G-pseudotyped SIVmac-GFP virus stocks. Different preparations of the SIVmac-GFP virus stock were used in the experiments. After 72 h, GFP-positive cells were analyzed by FACS. The results shown are representative of those obtained in three independent experiments.