Fig. 2.

Rab1a/b and Rab43 are required for infectious virus production. HeLa cells were transfected once (Rab1a/b) or twice (Rab43) with siRNA duplexes as detailed in Materials and Methods. At 48 h after the final transfection, cells were infected with HSV-1 (MOI, 5), and cell-associated virus was collected 20 hpi. Titers were established on Vero cells, and bars represent the means of three independent experiments, normalized to the control siRNA (A). Parallel samples of infected cells were collected 20 hpi, lysed, separated by SDS-PAGE, and analyzed by Western blotting (B and C). For panel C, cells were additionally transfected with a plasmid expressing Rab43-GFP.