Fig. 3.

Rab1a/b is required for transport of glycoproteins from the ER. (A) COS7 cells were cotransfected with a plasmid expressing TBC1D20-myc or TBC1D20RA-myc and HSV-1 gM-GFP (green) or gB (green). Eight hours posttransfection cells were fixed and stained with anti-myc (blue) and anti-calnexin (red). (B and C) HeLa cells were transfected with siRNA duplexes as described in the text. (B) Cells were infected with HSV-1ΔgE (MOI, 3), fixed at 8 hpi, and labeled with TGN46 (red) or calnexin (red) and gD (green). Bars, 10 μm. (C) Cells were infected with HSV-1 (MOI, 5), and 20 hpi cell pellets were lysed, separated by SDS-PAGE, and analyzed by Western blotting with antibodies to gD, gB, and gH.