Fig. 5.

Rab43 causes Golgi complex fragmentation but is not required for delivery of glycoproteins to the plasma membrane. (A and B) Cos7 cells were transfected with plasmids expressing RN-treRA-myc or RN-tre-myc and gM-GFP (A, upper panels) or gB (A, green, lower panels) or gD (B, all green), fixed 24 h posttransfection, and stained with antibodies to myc (blue) and TGN46 (red) or GM130 (red). (C) HeLa cells were transfected with siRNA and infected (MOI, 3) as detailed in the text. At 8 hpi cells were fixed and labeled with antibodies to gD (green, left panels) or gB (green, right panels) and TGN46 (red). Bars, 10 μm.