Fig. 6.

Analysis of glycoprotein localization after internalization from the plasma membrane in infected cells. (A to C) HeLa cells were cotransfected with plasmids expressing RN-tre or RN-treRA-myc (blue) (A) or RN-tre or RN-treRA-GFP (green) (B and C). At 24 h posttransfection cells were infected with HSV-1ΔgE (MOI, 3) for 8 h, whereupon anti-gD antibody was bound at 4°C for 1 h and subsequently incubated at 37°C for 60 min (A), 15 min (B), or 30 min (C). The cells were then fixed and labeled with appropriate secondary antibodies as well as anti-TGN46 (red) or anti-EEA1 (blue). For panel C, anti-gB antibody (blue) was used alongside anti-gD antibody.