Table 2.
rPIV5-P-T286E revertantsa
| Theoretical nucleic acids (amino acid) at position 286 | Nucleic acids found | Amino acid change | Frequency |
|---|---|---|---|
| GAA (E) | AAA | E → K | 9/26 |
| GAA (E) | GCA | E → A | 4/26 |
| GAA (E) | GTA | E → V | 2/26 |
| GAA (E) | GAC | E → D | 4/26 |
| GAA (E) | ACT | E → T | 1/26 |
| GAA (E) | Mixture | Mixture | 6/26 |
a
Three individual rescues were performed, and 26 plaques in total were examined. The supernatants of the infected cells were used for viral RNA extraction, RT-PCR, and sequencing. The differences in nucleic acid residues and amino acid residues are shown.