G protein-specific subtyping multiplex nested RT-PCR. (A) Amplicon from patient 1 (P1) (SA367859Pt06) next to a negative control (–) and the prototype subtype A- and B-positive-control strains. (B) Multiplex nested PCR products: lanes 1 to 5, clinical specimens; lane 6, negative control; lane 7, positive-control RSV A; lane 8, positive-control RSV B; lane 4, deletion mutant 2 (SA374801Pt06).