Fig. 1.

Diagram of the recombinant VHSV genomes. The pVHSV-ΔNV plasmid was constructed by deleting the complete NV gene from nucleotide positions 4561 to 4982 (with respect to the antiviral sense of the complete genome). The pVHSV-ΔATG plasmid was constructed by mutating the first and second ATG (58th nucleotide position from the first ATG) codons of the NV ORF to TGA codons. The pVHSV-NV (IHNV) plasmid was made by replacing the ORF of VHSV NV with the ORF of the NV gene of the IHNV 220-90 strain. (Nucleotide positions relative to the VHSV complete genome are given in parentheses. G/NV, gene junction between G and NV; NV/L, gene junction between the NV and L genes.)