Fig. 8.
Comparison of replication and apoptotic kinetics of chimeric rVHSV-NVIHNV and rVHSV. EPC cells (∼1 × 106) were inoculated with equal amounts of rVHSV and rVHSV-NVIHNV at an MOI of 5.0 PFU per cell. (A) The low-molecular-weight DNAs from cell lysates were extracted and analyzed by a DNA laddering assay. (B) Cell lysates were harvested and analyzed for the virus-specific proteins by immunoblotting. C, control. (C) Infected cells were freeze-thawed, and viruses were quantified by plaque assay at the indicated time points. (D) Caspase 3-like, caspase 8-like, and caspase 9-like activities at the indicated times postinfection. Average titers and standard deviations (error bars) for the three replicates are shown. All assays were performed as described in Materials and Methods.