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Nucleolar disruption during ASFV infection. Intranuclear detection of the nucleolar B-23 marker in Vero cells uninfected (NI) and infected at 4, 6, 8, and 12 h p.i. with 5 PFU of BA71V. A specific monoclonal anti-B23 antibody and a Cy2-conjugated anti-mouse antibody were used for immunofluorescence. Scale bars, 5 μm.
