Fig. 5.

Luminal cDNA synthesis and liposomal leakage depend on virus concentration. Increasing virus concentration results in increased cDNA synthesis up to a virus/lipid ratio of about 10⁻⁶ (<10 virions/nanocontainer); at higher ratios the cDNA signal decreases again. RNA transfer was measured in three independent assays as described for Fig. 3, but using HRV2 at different concentrations as indicated. Bands corresponding to the amplified cDNA were quantified by using ImageJ (solid black lines). Data on leakage of a fluorophore from receptor-decorated liposomes measured with chip electrophoresis as in (41) are included for comparison (dashed line). Note that the decrease of the cDNA signal with higher virus concentrations correlates with the decrease of intact (tight) liposomes.