Table 1.
Parameterb | Value for IFNc |
||
---|---|---|---|
IFNφ1 (native) | IFNφ2 (native) | IFNφ2 (iodine) | |
Data collection statistics (MAX laboratory) | |||
Energy (keV) | 14,199 | 13,565 | 8,331 |
Space group | P21 | P61 | P61 |
Unit cell lengths | |||
a, b, c (Å) | 48.8, 62.2, 51.3 | 84.2, 84.2, 45.2 | 84.1, 84.1, 45.2 |
α, β, γ (°) | 90.00, 91.42, 90.00 | 90.00, 90.00, 120.00 | 90, 90, 120 |
Mosaicity (°) | 0.83 | 0.21 | 0.24 |
Resolution (Å) | 50–2.09 | 50–1.49 | 50–2.2 (2.3–2.2) |
No. of unique reflections | 17,632 | 30,020 | 18,512 |
Redundancy | 3.5 (2.4) | 5.4 (4.0) | 5.5 (5.7) |
Completeness (%) | 96.3 (78.9) | 98.1 (84.9) | 99.9 (99.8) |
〈I〉/〈σ〉 | 12.4 (2.15) | 26.16 (4.7) | 8.2 (12.6) |
Rmerge (%) | 14 (35.6) | 5.5 (20.6) | 9.0 (13.2) |
Refinement statistics | |||
Rcryst (%) | 0.1813 | 0.1413 | |
Rfree (%) | 0.2372 | 0.1874 | |
Est. coord. error (Å) | 0.26 | 0.25 | |
Est. phase error (°) | 24.00 | 16.90 | |
RMSD from ideal | |||
Bond length (Å) | 0.008 | 0.005 | |
Bond angle (°) | 1.033 | 0.838 | |
Wilson B-factor (Å2) | |||
Protein | 24.51 | 19.42 | |
Water | 30.69 | 37.38 | |
Other ligands | 74.00 | ||
No. of atoms | 2,776 | 1,552 | |
No. of water molecules | 179 | 265 | |
No. of other molecules | 2 Ni | 0 | |
Ramachandran plot | |||
Favored (%) | 98.71 | 100 | |
Allowed (%) | 100 | 100 |
Statistics for data collection and phases are shown.
Est. coord. error, estimated coordinate error; RMSD, root mean square deviation.
The values for the interferons found by using the iodide derivative and native data set are shown. The values in parentheses are the values for the highest-resolution shells.