Table 1.
Statistics of crystallographic data for the zebrafish interferonsa
| Parameterb | Value for IFNc |
||
|---|---|---|---|
| IFNφ1 (native) | IFNφ2 (native) | IFNφ2 (iodine) | |
| Data collection statistics (MAX laboratory) | |||
| Energy (keV) | 14,199 | 13,565 | 8,331 |
| Space group | P21 | P61 | P61 |
| Unit cell lengths | |||
| a, b, c (Å) | 48.8, 62.2, 51.3 | 84.2, 84.2, 45.2 | 84.1, 84.1, 45.2 |
| α, β, γ (°) | 90.00, 91.42, 90.00 | 90.00, 90.00, 120.00 | 90, 90, 120 |
| Mosaicity (°) | 0.83 | 0.21 | 0.24 |
| Resolution (Å) | 50–2.09 | 50–1.49 | 50–2.2 (2.3–2.2) |
| No. of unique reflections | 17,632 | 30,020 | 18,512 |
| Redundancy | 3.5 (2.4) | 5.4 (4.0) | 5.5 (5.7) |
| Completeness (%) | 96.3 (78.9) | 98.1 (84.9) | 99.9 (99.8) |
| 〈I〉/〈σ〉 | 12.4 (2.15) | 26.16 (4.7) | 8.2 (12.6) |
| Rmerge (%) | 14 (35.6) | 5.5 (20.6) | 9.0 (13.2) |
| Refinement statistics | |||
| Rcryst (%) | 0.1813 | 0.1413 | |
| Rfree (%) | 0.2372 | 0.1874 | |
| Est. coord. error (Å) | 0.26 | 0.25 | |
| Est. phase error (°) | 24.00 | 16.90 | |
| RMSD from ideal | |||
| Bond length (Å) | 0.008 | 0.005 | |
| Bond angle (°) | 1.033 | 0.838 | |
| Wilson B-factor (Å2) | |||
| Protein | 24.51 | 19.42 | |
| Water | 30.69 | 37.38 | |
| Other ligands | 74.00 | ||
| No. of atoms | 2,776 | 1,552 | |
| No. of water molecules | 179 | 265 | |
| No. of other molecules | 2 Ni | 0 | |
| Ramachandran plot | |||
| Favored (%) | 98.71 | 100 | |
| Allowed (%) | 100 | 100 | |
a
Statistics for data collection and phases are shown.
b
Est. coord. error, estimated coordinate error; RMSD, root mean square deviation.
c
The values for the interferons found by using the iodide derivative and native data set are shown. The values in parentheses are the values for the highest-resolution shells.