Fig. 1.

HPV16 E6 binds NHERF-1 and promotes its degradation in in vitro assays. (A and B) C33A cell extracts were incubated with the different GST fusion proteins indicated and loaded onto SDS-PAGE gel. The amounts of NHERF-1 associated with the different GST E6 proteins were determined by immunoblotting using a specific anti-NHERF-1 antibody. One-tenth of the total cellular extract (60 μg) used in the GST pulldown assay (input) was used for SDS-PAGE. (C and D) In vitro-translated NHERF-1 was incubated at 30°C for 0, 1, or 2 h either alone or with in vitro-translated HPV16 or HPV18 E6 (C) or wild-type (WT) or ΔC mutant HPV16 E6 (D). The remaining targeted proteins were visualized by SDS-PAGE and autoradiography.