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. 2011 Aug;85(16):8208–8216. doi: 10.1128/JVI.00114-11

Fig. 6.

Fig. 6.

NHERF-1 levels are downregulated in HPV16-positive cervical cancer cell lines and in cervical lesions. (A) Protein extracts of different cell lines as indicated were prepared and analyzed by immunoblotting using anti-NHERF-1 and anti-β-actin antibodies. (B) SiHa and HeLa cells were transiently transfected with the indicated small interfering RNAs. After preparation of total protein extracts, NHERF-1, E6AP, p53, and α-actinin protein levels were determined by immunoblotting using specific antibodies. (C) Protein extracts from HeLa cells were incubated in the presence and absence of λ-PP and analyzed by immunoblotting using anti-NHERF-1 and anti-β-actin antibodies. (D) Sections of HPV16-positive cervical intraepithelial neoplasia grade III (CINIII) from two different women (a and b) were stained with a specific anti-NHERF-1 antibody. Arrows indicate the dysplastic regions. The images are shown at two magnifications (×10 and ×40).