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. Author manuscript; available in PMC: 2012 Oct 1.
Published in final edited form as: Biosci Rep. 2011 Oct 1;31(5):333–343. doi: 10.1042/BSR20100124

Figure 1. Brain-derived nuclear MeCP2 exists in multiple biochemically distinct pools.

Figure 1

(A) Chromatographic separation of crude rat brain nuclear extract by strong anion exchange (MonoQ resin) results in three distinct pools of MeCP2 as indicated by western blot using the MeCP2 7–18 antibody (Figure S1). The majority of MeCP2 does not bind the column (QF), while the bound MeCP2 elutes in two peaks, at 230mM NaCl (QB1/2) and 450mM NaCl (QB3). (B) MonoQ resin-bound fractions of MeCP2 were treated with (+) or without benzonase nuclease and tested for ability to re-bind the Mono Q. Western blot analysis for MeCP2 of MonoQ flow thru, wash, and 1000mM NaCl step elution shows benzonase treatment does not affect binding of MeCP2 to the Mono Q column. (C) Plasmid spiked MonoQ fractions treated with benzonase (+) or untreated (−) in parallel served as controls for benzonase treatment.