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. Author manuscript; available in PMC: 2012 Oct 1.
Published in final edited form as: Biosci Rep. 2011 Oct 1;31(5):333–343. doi: 10.1042/BSR20100124

Figure 4. Co-IPs confirmed MeCP2 associates with Prpf3 in vivo.

Figure 4

(A) Western blotting of a co-IP experiment from fractionated brain extract showing: a peak MeCP2 fraction (lane 1, 5% input), control IgG IP (lane 2), anti-MeCP2 IP (lane 3), and anti-MeCP2 IP treated with benzonase (lane 4), demonstrates that Prpf3 interacts with MeCP2 in the brain independent of nucleic acids. (B) Western blotting of a co-IP experiment from HA-MeCP2 transfected HT22 cells showing: whole cell extract (lane 1, 5% input), IgG IP (lane 2), anti-HA IP (lane 3) and anti-HA IP treated with benzonase (lane 4), demonstrates that Prpf3 interacts with MeCP2 cell culture independent of nucleic acids.