Table 1.

permanova results of Sample, Day, Temperature and Day × Temperature effects on 387 pairwise weighted and unweighted UniFrac distances. Bold text indicates tests where P< 0.05.

	df^a	SS^a	MS^a	Pseudo-F	P
Unweighted UniFrac
All Samples
Sample	5	26	5.1	25	0.001
Day	1	0.27	0.27	0.73	0.75
Temperature	3	0.64	0.21	0.56	1.0
Day × Temperature	3	0.66	0.22	0.57	1.0
Fecal
Sample	1	3.3	3.3	19	0.001
Day	1	0.26	0.26	1.09	0.24
Temperature	3	0.58	0.19	0.79	0.83
Day × Temperature	3	0.57	0.19	0.77	0.86
Soil
Sample	1	1.4	1.4	6.2	0.001
Day	1	0.27	0.27	1.11	0.15
Temperature	3	0.68	0.23	0.92	0.87
Day × Temperature	3	0.66	0.22	0.89	0.98
Skin
Sample	1	1.0	1.0	4.8	0.001
Day	1	0.32	0.32	1.4	0.02
Temperature	3	0.67	0.22	0.97	0.61
Day × Temperature	3	0.72	0.24	1.05	0.22
Weighted UniFrac
All Samples
Sample	5	14	2.8	301	0.001
Day	1	0.04	0.04	0.41	0.76
Temperature	3	0.05	0.02	0.15	1.0
Day × Temperature	3	0.04	0.01	0.12	1.0
Fecal
Sample	1	0.83	0.83	101	0.001
Day	1	0.06	0.06	2.28	0.107
Temperature	3	0.07	0.02	0.93	0.475
Day × Temperature	3	0.03	0.01	0.38	0.894
Soil
Sample	1	0.46	0.46	56	0.001
Day	1	0.04	0.04	2.07	0.11
Temperature	3	0.04	0.01	0.67	0.70
Day × Temperature	3	0.02	0.01	0.38	0.99
Skin
Sample	1	0.87	0.87	79	0.001
Day	1	0.02	0.02	0.51	0.53
Temperature	3	0.03	0.01	0.28	0.93
Day × Temperature	3	0.05	0.02	0.53	0.73

df=degrees of freedom: SS= sum of squares; MS= mean squares