Table 2.
Faith’s phylogenetic diversity (Faith’s PD) analysis of samples. Mean Faith’s PD and (SEM) are shown for individual sub-samples, and each temperature on both days. Temperature had a significant effect on Faith’s PD for Fecal sample 1 on Day 3 and was significantly greater on Day 14 for Fecal 1(P< 0.05 in both cases). The phylogenetic diversity of the remaining samples was unaffected by temperature or length of storage.
| Day 3 | Day 14 | |
|---|---|---|
| Fecal 1 | 12 (0.9) | 15 (0.7) |
| 20°C | 9.2 (0.4) | 15 (0.4) |
| 4°C | 12 (0.1) | 15 (0.8) |
| −20°C | 13 (0.3) | 15 (0.3) |
| −80°C | 13 (0.3) | 14 (0.4) |
| Fecal 2 | 7.7 (0.2) | 7.9 (0.3) |
| 20°C | 7.6 (0.3) | 8.1 (0.4) |
| 4°C | 7.7 (0.1) | 7.6 (0.3) |
| −20°C | 7.6 (0.2) | 8.2 (0.4) |
| −80°C | 7.8 (0.1) | 7.8 (0.1) |
| Skin 1 | 13 (1.5) | 13 (0.7) |
| 20°C | 12 (0.2) | 13 (0.7) |
| 4°C | 12 (0.2) | 14 (0.04) |
| −20°C | 13 (0.4) | 13 (1.3) |
| −80°C | 16 (2.3) | 12 (0.4) |
| Skin 2 | 8.2 (1.0) | 7.6 (0.7) |
| 20°C | 7.0 (0.1) | 8.7 (0.8) |
| 4°C | 8.1 (0.9) | 7.0 (0.1) |
| −20°C | 7.8 (1.2) | 7.8 (0.9) |
| −80°C | 9.7 (0.9) | 6.9 (0.2) |
| Soil 1 | 38 (1.0) | 39 (0.9) |
| 20°C | 37 (0.9) | 39 (1.2) |
| 4°C | 36 (1.3) | 39 (1.3) |
| −20°C | 39 (0.1) | 40 (0.8) |
| −80°C | 40 (0.5) | 40 (0.6) |
| Soil 2 | 43 (1.3) | 40 (0.5) |
| 20°C | 40 (0.6) | 40 (0.1) |
| 4°C | 43 (0.3) | 39 (0.6) |
| −20°C | 44 (1.4) | 39 (0.2) |
| −80°C | 45 (0.7) | 40 (0.8) |