Fig. 5.

Effect of DMSO on dual-readout F² assay performance and the stability of the competition assay. The interaction of Mcl-1 (62.5 nM) with TMR-Noxa peptide (125 nM) was carried out in the presence of an increasing amount of DMSO (0%–9%) at room temperature for 2 h. (A) The TR-FRET signals were measured and plotted against the increasing amount of DMSO. (B) The FP signal was measured and plotted against an increasing amount of DMSO. (C, D) The stability of the inhibitory effect of the nonlabeled Noxa peptide on the binding of TMR-Noxa peptide (125 nM) to Mcl-1 protein (50 nM) measured by TR-FRET readout (C) or FP readout (D) after 2 or 10 h incubation at room temperature. DMSO, dimethyl sulfoxide.