Fig. 7.

Gel filtration analyses of CD9 and mutant CD9 complexes. (A) U937 cells stably expressing CD9 or mutant CD9 were surface labeled with biotin and then lysed in 1% Brij 96. Lysates were fractionated using a sepharose CL6B gel filtration column and biotin-labeled CD9 was detected as described in the Materials and Methods. (B) U937 cells were metabolically labeled using ³H-palmitate, and then CD9 and mutant CD9 were fractionated and detected as described in the Materials and Methods. The sum of densitometry quantifications for each set of samples is 100%.