Unrooted, neighbor-joining tree of the predicted polar amino acid periplasmic binding proteins for selected organisms. The tree was reconstructed using the PHYLIP package (SEQBOOT, PROTDIST, NEIGHBOR, CONSENSE and FITCH programs). Nodes with bootstrap value exceeding 60% are marked by open circles. BS, B. subtilis; CA, Cl. acetobutylicum; Cpn, C.pneumoniae; DR, D. radiodurans; EC, E.coli; HI, H. influenzae; Rv, M. tuberculosis; TM, T. maritima. Experimentally established specificity of transporters is indicated in parentheses. Genes with candidate ARG boxes in upstream regions are shown in italic and in a larger font.