Table 1. Primers, 5′ nuclease probes, and characteristics of the qPCR assays run on the MFB.

		SAR11 16S rRNA gene	Marine crenarchaeal 16S rRNA gene	Synechococcus rbcL
qPCR primers and probes∧	Forward Primer	SAR11-433f CTCTTTCGTCGGGGAAGAAA (500 nM)	ARCHG1-334F AGATGGGTACTGAGACACGGAC (1000 nM)	RbcLfCAGACCACCCTCGGCTACAT (333 nM)
	Reverse Primer	SAR11-588R CCACCTACGWGCTCTTAAGC (1500 nM)	ARCHG1-554R CTGTAGGCCCAATAATCATCCT (500 nM)	RbcLrCCCAGTCCTGATCGAAGAAGTT (333 nM)
	5′ Nuclease Probe	TM519bR TTACCGCGGCTGCTGGCAC (200 nM)	TM519aRTTACCGCGGCGGCTGGCAC (400 nM)	TMrbcLTTCGTTCCTGAAGATCGCAGCCG (200 nM)
	Assay Reference	[6]	[5]	[44]
MFB	Δ fluorescence*	1000	1400	500
	NTC	No Amplification	No Amplification	No Amplification
	Slope	−3.8646	−3.5654	−3.1259
	Intercept	40.269	41.495	41.862
	R2	0.9949	0.9872	0.9473
	PCR efficiency	0.81	0.91	1.09
ABI7700	PCR efficiency	0.97	0.98	0.97

^∧final concentration in 30 µL reaction.

*at the end of a qPCR reaction run with the 10⁴ standard dilution.

PCR efficiency = 10^(−1/slope)−1.