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. 2011 Aug 1;6(8):e23046. doi: 10.1371/journal.pone.0023046

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Hong et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Putative SUMOylation sites of DEC1. (B) COS-7 cells were transfected with HA-tagged wild-type DEC1 or its mutants K159R, K279R or K159R/K279R (2K/2R) and Myc-tagged SUMO1, and then subjected to western blot with anti-HA antibody. (C) COS-7 cells were transfected with HA-tagged wild-type DEC1 or its mutant K159R/K279R (2K/2R) with or without Flag-tagged SENP1 or SENP1 mutant (mu) and then subjected to western blot with anti-HA antibody. *, p<0.05; **, p<0.01.