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. 2011 Aug 1;6(8):e23110. doi: 10.1371/journal.pone.0023110

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Zou et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) KYSE150/GFP-Aurora-A cells were exposed to 1 µM of Aurora-A kinase inhibitor and cellular proteins were collected 2 hours later. Western Blot was performed with antibodies to pThr288 on Aurora-A or total Aurora-A. DMSO was used as a negative control. (B) KYSE150/GFP-Aurora-A cells were exposed to Aurora-A kinase inhibitor or DMSO for 2 hours prior to treatment with CHX. Cells were harvested at the indicated time points and analyzed by Western Blot. (C) The amounts of AP-2α were calculated by densitometry and normalized to corresponding actin levels. The column diagram represents the amount of normalized AP-2α at each time point comparing with the original levels (0 h).