Figure 1. Intrinsic Requirement for STAT3 in T Cell-Dependent Colitis.

(A–E) CD4⁺CD45RB^hiCD25⁻ naive T cells from control (Stat3^fl/fl) or Stat3^−/− (Cd4 Cre; Stat3^fl/fl) mice were transferred into Rag2^−/− mice, which were monitored for evidence of colitis.

(A) Weight loss (percentage of initial weight at day 0) was calculated for each mouse over 9 weeks. Data show mean (±SEM) weight changes for each group (n = 4–5 mice) and are representative of four independent experiments.

(B and C) Colitis was assessed histologically at 9 weeks and severity was scored by markers of inflammation (Izcue et al., 2008). Each point represents an individual mouse and the data are pooled from three independent experiments. Unreconstituted Rag2^−/− mice and mice that received naive T cells with CD4⁺CD25⁺ Treg cells were included as controls.

(D and E) Systemic inflammation was assessed by measuring (D) spleen weights and (E) mean (±SEM) concentrations of inflammatory cytokines and chemokines in the serum. Data are representative of two independent experiments. ^*p < 0.05, ^**p < 0.005, ^***p < 0.0005.