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. Author manuscript; available in PMC: 2012 Aug 4.
Published in final edited form as: FEBS Lett. 2011 Jul 4;585(15):2488–2492. doi: 10.1016/j.febslet.2011.06.032

Fig. 1. Effectiveness of the silencing effects of iNOS shRNAs and the induction of iNOS shRNA production.

Fig. 1

A: A diagram showing the basic structure of the plasmids used in the study. B: Human 293FT cells were co-transfected with the plasmid pCMV-iNOS and various pCMV-shRNA plasmids indicated in the figure. RNA was prepared at 24 h after the transfection. Results are means ± S.D. (n = 3). ^ P < 0.05 compared with pCMV-N group. C: RASMCs were transfected with various plasmids indicated in the figure. Twenty four hours later, they were incubated with various concentrations of lipopolysaccharide for 12 h and then harvested for shRNA4 quantification. Results are means ± S.D. (n = 3). * P < 0.05 compared with piNOS-shRNA4 at 0 µg/ml lipopolysaccharide.