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. Author manuscript; available in PMC: 2012 Aug 15.
Published in final edited form as: Toxicol Appl Pharmacol. 2011 May 23;255(1):18–31. doi: 10.1016/j.taap.2011.05.012

Figure 2.

Figure 2

11-gene biomarker set in the mouse lung at 56 days post-exposure to MWCNT. Total RNA was extracted from the frozen stored lung tissue of mice that were exposed to different doses of MWCNT at 56 days post-exposure as indicated, followed by cDNA generation. Real-time PCR was performed to profile 63 lung cancer biomarker genes using LDA. Genes shown in the figure underwent significant changes in expression when compared to the negative control. A, Bcl2. B, Cav1. C, Ccdc99. D, Dhh. E, Gpx3. F, Msx2. G, Nos2. H, Pi3kr1. I, Ptch1. J, Wif1. K, Zak. L, 18S. Fold change was calculated after normalization to 18S and relative to control samples. Values given are mean ± SEM (p ≤ 0.05, n=8). * significantly different compared to control (unpaired t-tests).