Skip to main content
. Author manuscript; available in PMC: 2012 Aug 1.
Published in final edited form as: Cancer Res. 2011 Jun 8;71(15):5276–5286. doi: 10.1158/0008-5472.CAN-10-2160

Figure 2.

Figure 2

1,25(OH)2D3 induces G1 and G2/M cell cycle arrests and senescence in PrP/SC. (A) Cells were treated with 0.1% ethanol (EtOH) or 100 nM 1,25(OH)2D3 (1,25D3) for the indicated times. Each bar represents the mean of 4 replicate samples ± SEM. Statistical evaluations were determined by ANOVA with posthoc analysis by Scheffe’s F-test (* = p<0.05, ** = p< 0.005). (B) Representative images of a SA-β-gal assay in cells treated with increasing doses of 1,25(OH)2D3 every 48 hr for 96 hr. The positive control treatment was 100 nM doxorubicin (Dox). The negative control treatment was 100 nM doxorubicin at pH 7. Bars represent the percent of SA-β-gal-positive cells ± SEM (n = 10, * = p<0.05, Fisher’s LSD test).