In addition to the tumor cells, Her2/neu tumors contain cancer associated macrophages (F4/80), cancer associated fibroblasts (α-smooth muscle actin (aSMA)), lymphocytes (CD3), blood vessels (not shown), and extensive collagen deposition (trichrome). Also shown are IHC results of HER2/neu tumors for Timp1 and Vasp which were elevated in plasma from tumor-bearing mice. Additional proteins elevated in plasma from tumor-bearing mice are listed according to functional categories (boxes).