Figure 2.
MiR-193a and miR-193b expression levels and effects on tumorigenicity and invasive growth in breast cancer cell lines. A, Relative MiR-193a and miR-193b RNA levels in the indicated cell lines. Values for miR-193a and miR-193b in the non-transformed MCF-10A cells are arbitrarily set to 1. Based on comparable PCR amplification efficiencies determined experimentally, miR-193b appears to be approximately 3-fold more abundant than miR-193a in MCF-10A cells. B, Colony formation in soft agar of the indicated breast cancer cell lines transfected with miR-193a, miR-193b, or control miRNAs. C, Invasive growth (invading cell/field after wounding) of the indicated breast cancer cell lines transfected with miR-193a control miRNAs.