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. Author manuscript; available in PMC: 2012 Aug 11.
Published in final edited form as: Vaccine. 2011 Jun 25;29(35):6017–6028. doi: 10.1016/j.vaccine.2011.06.032

Fig. 2. Flow Cytometry gating strategy.

Fig. 2

On the left hand side, singlets were further gated on the lymphocytic population followed by a live/dead gate and selection of CD3+ cells. CD3+ cells were gated on CD4 or CD8 with further gating for central memory (CM), effector memory (EM), and total memory (TM) using CD28 and CD95. As an example, CD4 CM cells and CD8 TM cells were gated as shown for IL-17 secretion.