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. Author manuscript; available in PMC: 2012 Aug 6.
Published in final edited form as: Mol Cell Endocrinol. 2011 Jun 1;342(1-2):32–40. doi: 10.1016/j.mce.2011.05.025

Figure 2. Effect of 1,25D on PTHrP promoter activity mediated via P1, P2 or P3 in C4-2 and PC-3 cells.

Figure 2

Cells were transfected with the indicated promoter-driven luciferase reporter constructs plus Renilla luciferase construct, then treated with the indicated concentration of 1,25D or with ethanol (V, vehicle control). Luciferase activity was measured after 24 h. Empty vector control values were subtracted from the respective firefly and Renilla luciferase values. Values were then normalized to Renilla luciferase activity, and are expressed as the Firefly/Renilla ratio. P = promoter. Each bar is the mean ± SEM of three experiments. * = Significantly different from the vehicle control (P < 0.001).