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. Author manuscript; available in PMC: 2012 Aug 6.
Published in final edited form as: Mol Cell Endocrinol. 2011 Jun 1;342(1-2):32–40. doi: 10.1016/j.mce.2011.05.025

Figure 6. Effect of 1,25D on the PTHrP protein half-life.

Figure 6

PC-3 cells were treated with 1,25D (10−7 M) or ethanol (EtOH, vehicle control) for 48 h. The protein synthesis inhibitor CHX (150 µg/ml) was then added. At the indicated time points, lysates were prepared for Western blotting. (A) Representative Western blot showing decay of PTHrP levels in 1,25D- or ethanol-treated cells co-treated with CHX. (B) Densitometric analysis of the decay of PTHrP levels after CHX treatment in cells pre-treated with 1,25D or ethanol. Each point is the mean ± SEM of data from three independent experiments. The 0 time-point (at the time of CHX addition) is set at 100%. (C) Comparison of halflife of PTHrP in 1,25D- and ethanol-treated cells. The values were obtained from the data presented in (B). * = Significantly different from the vehicle control value (P < 0.001).