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. 2010 Jun 29;11:50. doi: 10.1186/1471-2199-11-50

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Copyright © 2010 Spanier et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Differential expression of aTub, GAPDH and MMP evaluated with REST using different normalization strategies. Program and genes used as reference genes for normalization: a) geNorm: Tbp/Xbp1/CAPON; b) NormFinder I: Tbp/Xbp1; c) NormFinder II: Xbp1/Stx16; d) the classical house-keeping genes Tbp/aTub/GAPDH; e) all genes, i.e.: Tbp/aTub/GAPDH/Stx16/Xbp1/CAPON. Boxes represent the interquartile range, or the middle 50% of observations. The dotted line represents the median gene expression. Whiskers represent the minimum and maximum observations.