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. 2011 Mar 31;14(1):20–27. doi: 10.4048/jbc.2011.14.1.20

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© 2011 Korean Breast Cancer Society

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Effect of ICI182780 (ICI) on propyl pyrazole triol (PPT)-induced claudin-6 (CLDN6) expression. (A) The effect of ICI on PPT-induced CLDN6 mRNA expression was determined by RT-PCR. Lane 1 and Lane 2 showed the expression of CLDN6 in control cells (C) and vacant vehicle control cells (VE) respectively. Lanes 3-5 showed that 5×10^-9 mol/L 17β-estradiol (E₂) and 10^-5 mol/L PPT for 24 hr induced CLDN6 expression respectively or collectively. Lane 6 shows that ICI significantly blocked the effect of PPT. (B) The Western blot assay revealed CLDN6 protein expression in the six groups. Lanes 3-5 showed that CLDN6 protein expression was significantly upregulated in MCF-7 cells treated with 17-β-estradiol or (and) PPT for 24 hr. Lane 6 shows that the effect of PPT could be blocked by ICI. (C) Immunofluoresce staining showed CLDN6 expression on the membranes of MCF-7 cells treated with 17β-estradiol or (and) PPT for 24 hr.

^*p<0.05, vs. MCF-7 cells (statistically significant).