Skip to main content
. Author manuscript; available in PMC: 2011 Aug 2.
Published in final edited form as: Reproduction. 2010 Nov 12;141(2):227–239. doi: 10.1530/REP-10-0334

Figure 2.

Figure 2

Effect of adhesion to ECM on Plau mRNA expression during trophoblast outgrowth. Blastocysts were cultured on BSA, fibronectin (FN), laminin (LN), or type IV collagen (Col) from GD 5 to GD 8. On GD 8, total RNA was extracted to estimate Plau or Gapdh mRNA, as in Fig. 1. (A) Representative gels of amplicons produced for Plau and Gapdh after 30 cycles of amplification. (B) Densitometric analysis of amplicon bands, normalized to Gapdh. Means±S.E.M. from three independent experiments are shown. *P<0.05, relative to culture on BSA.